In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls.

Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.

From the reviews: "This is a condensed and concise account of the various techniques used to study toll-like receptors, their downstream signaling pathways, and their role in the pathogenesis of disease. ? In addition to biologists and medical researchers, students, residents, and practitioners with an interest in the fields of bacteriology and immunology ? find this book interesting. ? It is adequately illustrated with black-and-white figures and color plates which readers will find useful." (Omer Iqbal, Doody's Review Service, July, 2009) "The book 'Toll-Like Receptors: Methods and Protocols' with 65 contributors, focuses on recent developments of TLRs investigations performed by many institutions including Universities, Technical Universities and Medical Schools in Europe and USA. ? Experimental tips and troubleshooting procedures are also included, which is invaluable for beginners in the field. ? an excellent manual for all investigators as well as teachers and advanced students of biological and medical sciences interested in modern immunology. ? fills an informational gap in very vital field of knowledge." (Elzbieta Walajtys-Rode, Acta Biochimica Polonica, Vol. 56, December, 2009) "This book is another protocols book in the successful Humana Press Methods in Molecular Biology series. It is specifically aimed at biologists and medical researchers active in the Toll-like receptor (TLR) research field or those who will develop an interest in TLR research in the future. ? this book has been well-targeted and specifically tailored to the researcher, but in a language that makes it accessible and easy to understand for final year undergraduate and post-graduate students." (Andrew Foey, Immunology News, November, 2009)