Amino acid analysis is widely used in biotechnology, biomedical, and food analysis laboratories. Amino Acid Analysis Protocols constitutes a major collection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Since results depend heavily on the quality of the sample, most contributors have devoted a section to sample preparation, particularly to the collection and storage of bodily fluids. A new method for desalting samples prior to hydrolysis is also provided. Each method is described in step-by-step detail to ensure successful experimental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems.
Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.
"Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis."-Doody's Health Sciences Book Review Journal
"...the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis."- Today's Life Science
"...This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories...This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques." - Clinical Chemistry
"Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids." - Journal of Chromatographic Science
