In the present work the basic aspects for the creation of the database were analyzed and an attempt is made for the required software development from the results obtained by sequencing the blood samples using 16 STR markers covering both dry and wet lab work. Programming languages essential for the creation of the data were to be examined and to develop the web application for the maintenance of the allelic data which otherwise represents the DNA database or DNA fingerprint identity (as every person has the unique DNA ) with user friendly interface having multi functionality for uploading the allelic data and personal information of the individuals as it gives individual identity, with a search option within the database for obtaining results of the query.