This two-volume set LNBI 16050-16051 constitutes the proceedings of the 12th International Conference on Bioinformatics and Biomedical Engineering, IWBBIO 2025, held in Canaria, Spain, during July 16-18, 2025.

The 57 full papers presented in these volumes were carefully reviewed and selected from 98 submissions. They were organized into the following topical sections:

Part I: Advances in Deep Learning in Bioinformatics and Bioengineering; Bioinformatics and Biomedical Applications; Biomarker Identification; Biomedical Computing; and Biomedical Engineering.

Part II: Biosensors and Data Acquisition; Emerging Trends and Innovations in E-Health; High Performance in Bioinformatics; Innovations in Cancer Research: The Role of Bioinformatics and Biomedical Engineering; Machine Learning in Bioinformatics and Biomedicine; and Recent Advances in Bioinformatics.